- Extinction Coefficient of Cyanmethemoglobin in various Spectrophotometers
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Ki Hong Kim, IK Whan Lee, Yong Myung Kim, Joo Sup Lee
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Korean J Cytopathol. 1968;2(2):143-147.
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 Abstract
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- Drabkin's cyanmethemoglobin method is acknowledged as the best available photometric procedure for the determination of total hemoglobin in blood.
In Korea standard hemoglobin (Acuglobin, Ortho Co.) has been not imported, so cyanmethemoglobin method in hemoglobinometry is not widely used in many hospitals which are equiped with spectrophotometer. Only a few hospitals are available for this method by using Fisher Hemophotometer.
Hemoglobin, however, can be calculated without standard solution by knowing extinction coefficient of cyanmethemoglobin in various photometers.
Authors present the extinction coefficient in Beckman DU, Beckman B, Coleman Jr. 6, and Spectronic 20.
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